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Syllabus for: PLSS 433 Intro to Agricultural Biotechnology

Spring 2004

Course Description: 

This course will cover the basic principles of plant and animal biotechnology using current examples; gene mapping in breeding, transgenic approaches to improve crop plants and transgenic approaches to improve animals will be considered.  Technology transfer from laboratory to marketplace will be considered.  An understanding of gene mapping, cloning, transfer, and expression will be derived.

 

Objectives: 

1.  To gain an understanding of the techniques and terminology of Biotechnology.

2.  To understand the applicability of Agricultural Biotechnology.

3.  To understand the gains attainable through Agricultural Biotechnology.

4.  To appreciate the nature of environmental and ethical concerns over Biotechnology.

 

Instructors:                                                     Guest Lecturers

David A. Lightfoot & Javed Iqbal, Ph.D.           Karen Jones, Ph.D., Todd Winters, L. Achenbach

Soy Ctr./Public Policy Inst. 113                         Ag 129

ga4082@siu.edu                                               kljones@siu.edu

453-1797                                                         453-1774

Office Hrs:                                                      Office Hrs:

MWF 9-11, 1-3                                               M 8-noon W3-5

or  by appointment                                            or by appointment

 

TA: Kay Cryder-Shopinski,  Plant and Soil Science, PPI Bldg B1  cryerk18@hotmail.com, Office Hrs: W 10-4. 

Lecture:                                  Mondays, 4-5:50 PM, Ag 114 

Lab:                                        Thursdays, 3-5:50 PM, Ag 114

 

Webpages:

 http://mccoy.lib.siu.edu:8900/SCRIPT/PLSS433/scripts/serve_home www.siuc.edu/~pbgc    

Password:                                ga4082             User ID: ga4082


 

Textbooks (in bookstore under PLSS): 

Molecular Biotechnology: Principles & Applications of Recombinant DNA, Glick & Pasternick—Required New 3rd edition

A Cartoon Guide to Genetics, Gonick--Optional.

Practical Applications of Plant Molecular Biology--Optional.

 

Molecular Biotechnology, Primrose--Reference (Previous Text)

Culture of Animals Cells: A Manual of  Basic Techniques, Freshney--Reference

Molecular Biology of the Gene, Watson--Reference

Genes VI, Levin--Reference

 

Grading:        

Quizzes: Ten point quizzes will be given during the first 10 minutes of each Monday lecture covering  material from the lectures and labs from the previous week since the last quiz.  The lowest two quizzes will be dropped.  Quizzes can not be made up.  A missed quiz will be counted as a dropped quiz. 

Exams: Three Take-home exams will be given during the semester.  Two midterms and one due during finals week.  10% will be taken off the top of exams each day that they are late.  Take-home exams should be typed unless permission is given by one of the instructors.  Do not plagiarize from text, reference                     materials, or webpages. 

Lab Reports: A number of lab reports (3-4) will be turned in during the semester.  Like take-home exams, 10% will be taken off the top of exams each day that they are late.  Legibility is important. 

Other Assignments:   A few other assignments in the course are probable. 

Incompletes:   Incompletes will only be given in special cases with written endorsement from a medical professional or university official.  Failing the course is not a reason for receiving an incomplete on its own.

 Grading Breakdown:           
                                                A=91-100%
                                                B=81-90%
                                                C=71-80%                
                                                D=61-70%
                                                F=
£60%

Exams                        45%
                                                Lab Reports              45%
                                                Quizzes, Misc.           10%
                                                                                   100%

 


 

 

PSGA/PLB/ANS/PLSS 433 Lecture & Lab Schedule

 

Date               Lecture Topic                                                                     Lecturer

Jan 26            Welcome Introduction and Review                                   Lightfoot

Jan 29             Lab: Lab Safety, Lab Books, & Pipette Calibration       Iqbal

Feb 2               Recombinant DNA Techniques: Cloning, PCR, etc.       Iqbal

Feb 5               Lab: Herbicide/Antibiotic Resistant Organisms             Iqbal

Feb 9               Recombinant DNA Techniques: Gene Probes                Iqbal

Feb 13             Lab:DNA Electrophoresis                                                Iqbal

Feb 16             Recombinant DNA Techniques: Molecular Markers     Iqbal

Feb 19             Lab: Transformation of Microbes                                     Iqbal

Feb 23             Plant Cell Culture & Propagation                                     Lightfoot

Feb 19             EXAM & Lab: Plant Cell Culture                                   Lightfoot

Mar 1              Microbiology Applications                                                Achenbach                 

Mar 4               Lab: Microbial Fermentation                                            Iqbal

Spring Break

Mar 15            Plant Cell Culture & Propagation                                     Lightfoot

Mar 18            Lab: Plant Transformation                                                Lightfoot

Mar 22           Transgenic Plants                                                                Lightfoot

Mar 25            Lab: DNA Analysis                                                             Lightfoot

Mar 29            Molecular Breeding: Plants & Animals                             Lightfoot

Apr 1               Lab: Molecular Markers for Breeding                                Lightfoot

Apr 5               Molecular Breeding: Plants & Animals                              Lightfoot

EXAM

Apr 8               Lab: More Molecular Markers for Breeding                       Lightfoot

Apr 12            Mammalian Cell Culture & Applications                             Jones

Apr 15            Lab: Mammalian Cell Culture                                             Jones

Apr 19            Antibodies & Applications                                                     Winters

Apr 22            Lab: Progesterone RIA                                                          Winters

Apr 26             Transgenic Animals & Applications                                     Jones

Apr 29             Lab:    In Vitro Embryo Production and Cryogenics          Jones

Apr 28             Environmental, Social, and Ethical Issues                           Lightfoot

Apr 31             Lab:    Wrap-Up Lab/Evaluations                                        Instructors

May 6             FINAL EXAM due

 

Friday lecture will be ad hoc, used to make up missed Monday classes or attend the MBMB seminar (LS3 157) when Biotechnology topics are presented.

 

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