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Syllabus for:
PLSS 433 Intro to Agricultural
Biotechnology
Spring 2004
Course Description:
This course will cover the basic principles
of plant and animal biotechnology using current examples; gene mapping in
breeding, transgenic approaches to improve crop plants and transgenic
approaches to improve animals will be considered. Technology transfer
from laboratory to marketplace will be considered. An understanding of
gene mapping, cloning, transfer, and expression will be derived.
Objectives:
1. To gain an
understanding of the techniques and terminology of Biotechnology.
2. To understand the
applicability of Agricultural Biotechnology.
3. To understand the
gains attainable through Agricultural Biotechnology.
4. To appreciate the
nature of environmental and ethical concerns over Biotechnology.
Instructors:
Guest Lecturers
David A. Lightfoot & Javed Iqbal,
Ph.D. Karen Jones, Ph.D., Todd Winters, L. Achenbach
Soy Ctr./Public Policy Inst.
113 Ag 129
ga4082@siu.edu kljones@siu.edu
453-1797 453-1774
Office Hrs:
Office Hrs:
MWF 9-11,
1-3 M 8-noon W3-5
or by
appointment or by appointment
TA: Kay Cryder-Shopinski,
Plant and Soil Science, PPI Bldg B1
cryerk18@hotmail.com, Office Hrs: W 10-4.
Lecture:
Mondays, 4-5:50 PM, Ag 114
Lab:
Thursdays, 3-5:50 PM, Ag 114
Webpages:
http://mccoy.lib.siu.edu:8900/SCRIPT/PLSS433/scripts/serve_home
www.siuc.edu/~pbgc
Password:
ga4082 User ID: ga4082
Textbooks (in
bookstore under PLSS):
Molecular
Biotechnology: Principles & Applications of Recombinant DNA, Glick &
Pasternick—Required New 3rd edition
A Cartoon Guide to
Genetics, Gonick--Optional.
Practical Applications
of Plant Molecular Biology--Optional.
Molecular
Biotechnology, Primrose--Reference (Previous Text)
Culture of Animals
Cells: A Manual of Basic Techniques, Freshney--Reference
Molecular Biology of
the Gene, Watson--Reference
Genes VI,
Levin--Reference
Grading:
Quizzes: Ten point quizzes will be given during the first 10 minutes of each
Monday lecture covering material from the lectures and labs from the
previous week since the last quiz. The lowest two quizzes will be
dropped. Quizzes can not be made up. A missed quiz will be counted as a
dropped quiz.
Exams: Three Take-home exams will be given during the semester. Two midterms
and one due during finals week. 10% will be taken off the top of exams
each day that they are late. Take-home exams should be typed unless
permission is given by one of the instructors. Do not plagiarize from
text, reference materials, or webpages.
Lab Reports:
A number of lab reports (3-4) will be turned in during the semester. Like
take-home exams, 10% will be taken off the top of exams each day that they
are late. Legibility is important.
Other
Assignments: A few other
assignments in the course are probable.
Incompletes:
Incompletes will only be given
in special cases with written endorsement from a medical professional or
university official. Failing the course is not a reason for
receiving an incomplete on its own.
Grading
Breakdown:
A=91-100%
B=81-90%
C=71-80%
D=61-70%
F=£60%
Exams
45%
Lab Reports 45%
Quizzes, Misc. 10%
100%
PSGA/PLB/ANS/PLSS
433 Lecture & Lab Schedule
Date Lecture
Topic
Lecturer
Jan
26 Welcome Introduction and
Review Lightfoot
Jan
29 Lab: Lab Safety, Lab Books, & Pipette Calibration Iqbal
Feb
2 Recombinant DNA Techniques: Cloning, PCR, etc. Iqbal
Feb 5 Lab:
Herbicide/Antibiotic Resistant Organisms Iqbal
Feb
9 Recombinant DNA Techniques: Gene
Probes Iqbal
Feb
13
Lab:DNA Electrophoresis
Iqbal
Feb
16 Recombinant DNA Techniques: Molecular Markers
Iqbal
Feb
19
Lab: Transformation of Microbes Iqbal
Feb
23 Plant Cell Culture &
Propagation Lightfoot
Feb
19 EXAM & Lab: Plant Cell Culture
Lightfoot
Mar
1 Microbiology Applications
Achenbach
Mar
4 Lab:
Microbial Fermentation Iqbal
Spring Break
Mar
15 Plant Cell Culture &
Propagation Lightfoot
Mar
18 Lab: Plant Transformation Lightfoot
Mar
22 Transgenic
Plants
Lightfoot
Mar
25 Lab: DNA Analysis
Lightfoot
Mar
29 Molecular Breeding: Plants &
Animals Lightfoot
Apr
1 Lab: Molecular Markers for Breeding
Lightfoot
Apr
5 Molecular Breeding: Plants &
Animals Lightfoot
EXAM
Apr
8 Lab: More Molecular Markers for Breeding
Lightfoot
Apr
12 Mammalian Cell Culture &
Applications Jones
Apr
15 Lab: Mammalian Cell Culture
Jones
Apr
19 Antibodies & Applications
Winters
Apr
22 Lab: Progesterone RIA
Winters
Apr
26 Transgenic Animals & Applications
Jones
Apr
29 Lab: In Vitro Embryo Production and Cryogenics
Jones
Apr
28 Environmental, Social, and Ethical
Issues Lightfoot
Apr
31 Lab:
Wrap-Up Lab/Evaluations
Instructors
May
6 FINAL EXAM due
Friday lecture will be ad hoc, used to make up missed Monday classes or
attend the MBMB seminar (LS3 157) when Biotechnology topics are presented.
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