WPCPD n.fr>%C _|@ktna$$}aV 0` 0h 0bl 0H 0 0H 0H 0x* 0H 0L 06 08 0x: 0 0 0 0 0 0 0 0@ 0t" 0" 0# 0|$ 0|% 0% 0|& 0' 0( 0$) 0* 0+ 0L, 03. 0/ 0/ 00 01 02 03 0a4 0C5 0 6 06 07 0a8 0*9 0z9 0j: 00; 0; 0< 0= 0Rp> 0Z?AnX,A 0rB#!BNDw D4#D7D m9D<6X9`("Courier NewTT'   2 (david lightfoot 0 .   h~~:Default ParaDefault Paragraph FontXXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTOT~ :endnote textendnote text  XXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTO  ^:endnote refeendnote referenceXXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTOV~ :footnote texfootnote text  XXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTO  `:footnote reffootnote referenceXXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTOL~~6Document 8Document 8XXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTOL6Document 4Document 4          L~~6Document 6Document 6XXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTOL~~6Document 5Document 5XXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTOL6Document 2Document 2        L~~6Document 7Document 7XXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTOP~~8BibliogrphyBibliogrphyXXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTOP\K 8Right Par 1Right Par 1  ;1X` hp x (#;        5+ ` hp x (#5  P\K 8Right Par 2Right Par 2  ;1X` hp x (#;        5+ ` hp x (#5  L6Document 3Document 3        P\K 8Right Par 3Right Par 3  ;1X`  hp x (#;        5+ ` hp x (#5  P\K 8Right Par 4Right Par 4  ;1X` hp x (#;        5+ ` hp x (#5  P\K 8Right Par 5Right Par 5  ;1X` hp x (#;        5+ ` hp x (#5  P\K 8Right Par 6Right Par 6  ;1X` hp x (#;        5+ ` hp x (#5  P\K 8Right Par 7Right Par 7  ;1X` hp x (#;        5+ ` hp x (#5  P\K 8Right Par 8Right Par 8  ;1X` hp x (#;        5+ ` hp x (#5  LhW 6Document 1Document 1&    ;1X` hp x (#;        5+ ` hp x (#5'    D~~2Doc InitDoc InitXXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTOH4Tech InitTech Init        P_N 8Technical 5Technical 5  ;1X` hp x (#;        5+ ` hp x (#5  P_N 8Technical 6Technical 6  ;1X` hp x (#;        5+ ` hp x (#5  P8Technical 2Technical 2        P8Technical 3Technical 3        P_N 8Technical 4Technical 4  ;1X` hp x (#;        5+ ` hp x (#5  P8Technical 1Technical 1        P_N 8Technical 7Technical 7  ;1X` hp x (#;        5+ ` hp x (#5  P_N 8Technical 8Technical 8  ;1X` hp x (#;        5+ ` hp x (#5  @ 0HeadingHeading  8.` hp x (#8XXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTO5+ ` hp x (#5  H\K 4Right ParRight Par  ;1X` hp x (#;        5+ ` hp x (#5  L_N 6SubheadingSubheading  ;1X` hp x (#;        5+ ` hp x (#5  P~~8Bullet ListBullet ListXXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTO0\K (1 11 1  ;1X` hp x (#;        5+ ` hp x (#5  0\K (1 21 2  ;1X` hp x (#;        5+ ` hp x (#5  0\K (1 31 3  ;1X`  hp x (#;        5+ ` hp x (#5  0\K (1 41 4  ;1X` hp x (#;        5+ ` hp x (#5  0\K (1 51 5  ;1X` hp x (#;        5+ ` hp x (#5  0\K (1 61 6  ;1X` hp x (#;        5+ ` hp x (#5  0\K (1 71 7  ;1X` hp x (#;        5+ ` hp x (#5  0\K (1 81 8  ;1X` hp x (#;        5+ ` hp x (#5  88K ,toc 1toc 1   (#        5+ ` hp x (#5  88K ,toc 2toc 2   ` (#        5+ ` hp x (#5  8;K ,toc 3toc 3  ` (#        5+ ` hp x (#5  8;K ,toc 4toc 4   (#        5+ ` hp x (#5  8;K ,toc 5toc 5  h(#        5+ ` hp x (#5  88K ,toc 6toc 6   (#        5+ ` hp x (#5  8! ,toc 7toc 7            88K ,toc 8toc 8   (#        5+ ` hp x (#5  88K ,toc 9toc 9   (#        5+ ` hp x (#5  @;K 0index 1index 1  ` (#        5+ ` hp x (#5  @8K 0index 2index 2   ` (#        5+ ` hp x (#5  P5K 8toa headingtoa heading   (#        5+ ` hp x (#5  @~ 0captioncaption  XXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTO  ^~~:_Equation Ca_Equation CaptionXXXO<6X9`("Courier NewTTO        O<6X9`("Courier NewTTO3|x<6X9`("Courier NewTTd6X@DQ@<6X9`("Courier NewTTXXx6X@DQX@(JQ$  HP LaserJet 4,,,,,,0Duv(:iU @Q!   _B.` hp x (#XBX'X  _ _ԀRegistrationofEightRecombinantInbredLinesfromEssexby  ForrestwithResistancetoSoybeanSuddenDeathSyndrome(SDS)andCystNematoderace3.  p ̀M.E.Schmidt,P.T.GibsonandD.A._Lightfoot_*. p   ̀Dept.ofPlantandSoilScience,MolecularScienceProgram,πSouthernIllinoisUniv.,Carbondale,IL62091.Abbreviations:CI,cystindex;DI,diseaseincidence;DS,diseaseseverity;_QTL_,quantitativetraitloci;_RAPD_,randomamplifiedpolymorphicDNA;_RFLP_,restrictionfragmentlengthpolymorphism;_RIL_,recombinantinbredline.򀀀 0 " M.E.Schmidt,P.T.GibsonandD.A._Lightfoot_.Dept.ofPlantandSoilScience,MolecularScienceProgram,SouthernIllinoisUniv.,Carbondale,IL62091.ReceivedOctoberX,1995.Accepted.*Correspondingauthor.EmailGA4082@SIUCVMB.SIU.EDU.  p&!* _ԀEightRecombinantInbredLinesofsoybean[Glycinemax(L.)  Merr.]fromEssexbyForrestwithResistancetoSoybeanSuddenDeathSyndrome(SDS)andCystNematoderace3weredevelopedbytheDept.ofPlantandSoilScience,SouthernIllinoisUniv.,Carbondale,IL62091andreleasedin1994.EachRILwasderivedfromthe'Essex'by'Forrest'(ExF)F5derivedpopulationofsoybeanrecombinantinbredlines(RILs)thatwasconstructedbycrossingEssex(SmithandCamper,1973)withForrest(HartwigandEpps,1973)andwasadvancedtotheF5asdescribed(Hnetkovskyetal.,1996).̀TheeightRILswereallsignificantlymoreSDSresistantthanForrestbyDI(Hnetkovskyetal.,1996).Table1describesthemarkersbywhichtheQTLconditioningSDSDIcanbeselected.ThemeanSDSDSandyieldratinginSDSpathogeninfestedfieldenvironmentsisshown.RootrotcausedbytheSDSpathogen,Fusariumsolani(Mart.)Sacc.f.sp.phaseoli(Burk.)Snyd.&Hans.,typeA(Rupe,1989;Roy,1989,O'DonnellandGray,1995)wasdeterminedincornmeal/sandgreenhouseassays(O'DonnellandGray,1995;Tortoetal.,1996).Manganese(Mn)toxicitychlorosisratingwasdeterminedbyhydroponics(Kiloetal.,1996).SCNresistanceandCIweredeterminedasdescribed(Changetal.,1996,Njitietal.,1996).̀SeedsoftheRILsmaybeobtainedfromtheDept.ofPlantandSoilScience,SouthernIllinoisUniv.,Carbondale,IL62091foratleastfiveyears.ReferencesandNotesChangS.J.C.,T.W.Doubler,V.Kilo,R.Suttner,J.Klein,M.E.Schmidt,P.T.GibsonandD.A.Lightfoot.1996.LociunderlyingFieldResistancetoSoybeanSuddenDeathSyndrome(SDS)andCystNematodeMappedinRecombinantInbredLines.CropScience(submitted).0....Gibson,P.T.,M.A.Shenaut,V.N.Njiti,R.J.SuttnerandO.Myers,Jr.1994.Soybeanvarietalresponsetosuddendeathsyndrome.p.2040.InD.Wilkinson(ed.)Proc.TwentyfourthSoybeanSeedRes.Conf.,Chicago,IL67Dec.,Am.SeedTradeAssoc.,WashingtonDC.0....Hartwig,E.E.andJ.M.Epps.1973.RegistrationofForrest..soybeans.CropSci.13:287.0....Hnetkovsky,N.,S.J.C.Chang,T.W.Doubler,P.T.GibsonandD.A.Lightfoot.1996.GeneticMappingofLociUnderlyingFieldResistancetoSoybeanSuddenDeathSyndrome(SDS).CropScì36(2):(inpress).0....Kilo,V.andD.A.Lightfoot.1996.LociunderlyingResistancetoManganesetoxicityMappedinSoybeanRecombinantInbredLines.CropScience(submitted).Njiti,V.N.,M.A.Shenaut,R.J.Suttner,M.E.SchmidtandP.T.Gibson.1995.Segregationpatternforsoybeansuddendeathsyndrome(SDS)responseinPyramidxDouglasandtheinfluenceofsoybeancystnematode(SCN)resistance.CropSci.SubmittedMarch,1995.0....O'Donnell,K.andL.E.Gray.1995.Moleculartoolsforthe..detectionandrapididentificationofthesoybeansuddendeathsyndromepathogen,Fusariumsolanif.sp.phaseoli:PhylogeneticsandspeciesspecificPCRprimers.Molec.PlantMicr.Inter..Inpress.Roy,K.W.,G.W.Lawrence,H.H.Hodges,K.S.McLeanandJ.F.Killebrew.1989.Suddendeathsyndromeofsoybean:FusariumsolaniasincitantandrelationofHeteroderaglycinestodiseaseseverity.Phytopath.79:191197.Rupe,J.C.1989.FrequencyandpathogenicityofFusariumsolanirecoveredfromsoybeanswithsuddendeathsyndrome.PlantDis.73:581584.0....Smith,T.J.andH.M.Camper.1973.RegistrationofEssexsoybeans.CropSci.13:495.Torto,T.,Njiti,V.,ChangS.J.C.,T.W.Doubler,V.Kilo,R.Suttner,J.Klein,M.E.Schmidt,P.T.GibsonandD.A.Lightfoot.1996.LociunderlyingFieldandGreenhouseResistancetoSoybeanSuddenDeathSyndrome(SDS)MappedinRecombinantInbredLines.CropScience(submitted).    \R'3+ \J@X` hp x (#%'0*,.JO<6X9`("Courier NewTTOTable1.Markergenotypes,meanagronomicperformance,meanSDSresistanceandSCNresistanceoftheEssexbyForrest(ExF)RILsthatweremoreresistanttoSDSthanForrest(P<0.05)basedonSDSdiseaseincidence(DI).򀀀󀀀򀀀Linkagegroup'QTL󀀀Harvest̀SDS1G1C22G1NSDSYieldRootSCNSCNmaturityHeightLodgingLineDIG13I03O05K455E04E02C01F04DSIS(kg1haRotIPclassEssex(d)(cm)score#򀀀󀀀ExF784.4+-+-++-1.114.64120500.2R4.5361.2ExF597.2+++-++++1.27.93870590.8R1.4412.3ExF448.2+o+++++1.310.03760754.0S3.4362.0Forrest16.5++++++1.27.83760720.2R7.3392.7Essex58.9++1.618.23870654.0S0.0341.3򀀀̀'basedon(2,5),markersarepublicallyavailablefromBiogeneticServices(Brookings,SD)andOperonTechnologies(Alameda,CA).Presenceofthebeneficialalleleatthemarkeris+,absenceisandnotscoredunequivocallyiso.̀(Infectionseverity(IS)meanoverthegrowingseason(6)̀Rootrotexpressedaspercentageofuninfestedcontrol100((treatedwt/controlwt)100)wasevaluatedin..agreenhouseassay(after7).̀SCNrace3indexofparasitism(IP)expressedona05scaleresistanttosusceptibleinagreenhouse..assay(8).̀#Lodgingwasratedona0tofivescale.Table1.Markergenotypes,meanagronomicperformance,meanSDSresistanceandSCNresistanceoftheExFRILsthatweremoreresistanttoSDSthanForrest(P<0.05).򀀀󀀀LinkageGroup'QTLSDSYieldHarvest̀SDSG1C2G2N1RootSDSwithSDSSCNSCNMaturityHeightLodgingLineDIG13(I03O05K455E04E02C01F04RotDSkg1hIPClassEssex(d)(cm)Score#򀀀󀀀̀784.4+-+-++-501.141200.2R4.5361.2̀597.2+++-++++591.238700.8R1.4412.3̀377.8++++++o+741.238500.2R3.7341.4̀448.2+o+++++751.337604.0S3.4362.0̀578.8+++o++++711.338900.0R2.2402.2̀238.9++++-+++371.139600.0R1.8312.5̀679.8+++o+++411.239200.2R2.9381.6̀4710.6++++--+-91.237150.6R1.1342.0̀F16.5++++++721.237600.2R7.3392.7̀E58.9++651.638704.0S0.0341.3򀀀̀'RAPDprimers(Operon,Alameda,CA)andanRFLPmarker(K455D1).̀(Presenceofthebeneficialalleleatthemarkeris+,absenceisandnotscoredunequivocallyiso.̀Rootrotexpressedaspercentageofuninfestedcontrol100((treatedwt/controlwt)100)wasevaluatedin..agreenhouseassay.̀SCNrace3indexofparasitism(IP)expressedona05scaleresistanttosusceptibleinagreenhouse..assay.̀#Lodgingwasratedona0tofivescale.TheRILExF78hasbeenselectedforreleasebecauseitcontainsbeneficialallelesforresistancetoSDS(F.solani)thatderivefromForrest(3QTL)andEssex(1QTL).ThereisnoothercultivarthathasbeenreleasedwiththesefourQTLincoupling.TheQTLcanbeselectedwiththeDNAmarkersdescribedinHnetkovskyetal.,(1996)andChangetal.,(1996;1997).